气相色谱法分析柱状田头菇子实体中农药残留量

Determination of Chlorpyrifos,Cypermethrin and Deltamethrin Residues in Agrocybe cylindracea Fruit Bodies Using Gas Chromatography

本研究以柱状田头菇(Agrocybe cylindracea,俗称杨树菇)为食用菌子实体样本,以有代表性的毒死蜱、氯氰菊酯和溴氰菊酯3种农药为检测目标,建立1种利用气相色谱对食用菌子实体中多种农药残留量进行检测分析的方法。所建方法的色谱条件为:Agilent 6890N气相色谱仪,63 Ni-ECD检测器;HP-5毛细管柱(30m×0.32mm×0.25μm);高纯氮气(纯度>99.999%)为载气,流速1.5mL/min;进样口温度为250℃;检测器温度为280℃;柱前压为72psi,尾吹60mL/min;柱升温程序为初始柱温150℃,恒温3min后以6℃/min的速率升温至270℃,恒温25min后,不分流进样,进样量为1.0μL。方法学考察结果表明,该方法灵敏度和准确度高、加样回收率和变异系数符合《农药残留试验准则》相关要求;利用该法对柱状田头菇子实体样品农药残留进行检测的数据稳定、误差小。初步表明,建立的气相色谱方法适合用于柱状田头菇子实体中多种农药残留量的检测分析。

A simple and sensitive gas chromatography（GC）-based method for the accurate determination of chlorpyrifos,cypermethrin and deltamethrin residues in Agrocybe cylindracea fruit bodies is described.Pesticide recovery rates from homogenized A.cylindracea fruit bodies treated with different concentrations of chlorpyrifos,cypermethrin and deltamethrin ranged from 77.5%-86.3%,83.6%-86.4% and 85.3%-108.6%,respectively.Detection limits for chlorpyrifos,cypermethrin and deltamethrin were 2.3 ng,6.7 ng and 0.82 ng respectively,and the minimum concentrations detectable in mushroom fruit bodies were 0.87,2.9 and 0.3 μg/kg fresh weight,respectively.