CXCR1/CXCR2受体拮抗剂(G31P)对人前列腺癌增殖的体内外实验研究

Inhibition study of G31P,a chemokine receptor CXCR1/2 antagonist,in proliferation of human prostate cancer cells in vivo and in vitro

目的:探讨CXCR1/CXCR2受体拮抗剂-G31P对人前列腺癌细胞PC-3增殖的体内外抑制作用。方法:采用CCK-8法研究不同浓度G31P对PC-3细胞体外增殖的抑制作用。建立体内绿色荧光蛋白(Green fluorescent protein,GFP)标记人雄激素非依赖性前列腺癌细胞PC-3裸鼠原位移植瘤模型,观察G31P对裸鼠前列腺癌原位移植瘤的体积、重量的影响。结果:CCK-8结果显示100 ng/ml G31P与对照组相比分别作用1、3 d和5 d差异具有统计学意义(1 d P=0.007、3 d P=0.001、5 d P=0.028,均为P<0.05)。前列腺癌PC-3细胞裸鼠模型体内实验显示,与对照组(100μl N.S)相比,G31P处理组(0.5 mg/kg)从给药第18天起能显著抑制前列腺肿瘤的体积(P=0.026,P<0.05);与对照组相比G31P处理组在抑制前列腺肿瘤重量方面有明显作用(P=0.027,P<0.05)。结论:G31P体内外实验均能抑制人雄激素非依赖性前列腺癌细胞系PC-3的增殖。

Objective：To investigate the effect of G31P on the proliferation of the prostate cancer in vivo and in vitro.Methods：The inhibition effect of G31P on the proliferation of PC-3 cell was investigated by CCK-8 assay in vitro.The effect of G31P on the volume and weight of human prostate tumor of nude mice was observed by building orthotopic transplantation tumor model of human androgen-independent prostate cancer PC-3（GFP-labeled） in nude mice.Results：CCK-8 assay found statistically significant difference in inhibiting the proliferation of PC-3 cells between the 100 ng/ml G31P and control group for 1 day（P0.01）,3 days（P≤0.01） and 5 days（P0.05）.G31P treatment after the 18th day of administration inhibited significantly the prostate tumor volume compared with control group（P0.05）,and this inhibition was more obvious（P0.01） after the 24th day of administration in nude mice in vivo.A significant difference was observed between G31P treatment group（P0.05） and the control group in prostate tumor weight.Conclusion：G31P can inhibit the proliferation of the prostate cancer in vivo and in vitro.