高效芘降解细菌的筛选、鉴定及其基本特性研究

Isolation identification of highly efficient degradation bacteria of pyrene and its basic characteristics

以多环芳烃为唯一碳源梯度驯化,从焦化废水底泥中分离筛选出2株疏水性高效芘降解细菌CY4和HY7.通过16SrDNA基因序列分析,初步鉴定CY4为杀鲑气单胞菌(Aeromonas salmonicida),HY7为施氏假单胞菌(Pseudomonas stutzeri).降解性能试验结果表明,这两株菌可利用底物谱较为广泛,包括水杨酸等多环芳烃转化产物,吲哚等杂化芳烃,多环芳烃和葡萄糖;其中CY4菌对芘的5d降解率达35.40%,HY7菌对芘的5d降解率达36.32%.

Two highly efficient degradation hydrophobic strains were successfully isolated and screened from the bottom sludge of coke-plant waste water by the PAH-sole-carbon source gradient concentration domestication.According to the analysis of its 16S rDNA gene sequence,CY4 strain was identified as Aeromonas salmonicida which was a new strain in PAHs biodegradation reports to the best of our knowledge,while HY7 was identified as Pseudomonas stutzeri which was seldom reported.The results indicated that the two strains could utilize extensive substrates,including PAHs intermediates such as salicylic acid,hybrid aromatic hydrocarbon such as indole,PAHs and glucose.And the five days pyrene degradation rate was up to 35.40% by CY4 strain and up to 36.32% by HY7 strain.