家蚕丝蛋白基因在不同发育时期表达量的变化

Changes of Fibroin Gene Expression during the Different Development Period form Silkworm

本文采用半定量RT-PCR方法,检测了家蚕4～5龄期丝腺中5个丝蛋白基因(Fib-H、Fib-L、Ser-2短片段、Ser-2长片段、Ser-3)在不同发育时期表达量的变化。结果表明,Fib-H、FIB-L及Ser-3基因的表达规律相似,总体上4龄期表达量明显低于5龄,但4龄眠期均有明显表达,从5龄起蚕以后表达量逐渐上升,至熟蚕或熟蚕后2d达到高峰,此后迅速下降;Ser-2基因两个转录本的表达规律基本一致,与以上3个基因相比,Ser-2基因在4龄期存在相对较高的表达量,但在4龄眠期表达量明显降低,从5龄起蚕以后表达量逐渐上升,Ser-2短片段和长片段分别从5龄96h和144h之后表达量开始降低,到熟蚕期基本达到最低值。

RT-PCR technology is used to detect expression of five fibroin genes（Fib-H、Fib-L、Ser-2 long fragment、Ser-2 short fragment、Ser-3）from silkworm during the 4th-instar and 5th-instar. The result shows that the expression patterns of Fib-H、FIB-L、Ser-3 were similar. On the whole,the expression of the there genes during the 4th-instar was lower than that during 5th-instar. From the beginning of 5th-instar, the expression was gradually going up and reached the peak at maturing silkworm, since then rapidly declined. The expression patterns of the two transcripts of Ser-2 were similar. The expression of the two transcripts was relatively high in the 4th-instar, obviously dropped at molting stage and was gradually going up from the beginning of 5th-instar. The short fragment of Ser-2 and the long fragment separately began to decline from the 96h and 144 h of the 5th-instar, since then reached the valley at matured silkworm.