摘要
ISSR标记技术是在SSR基础上发展起来的一项新的标记技术,其引物设计简单,多态性高,重复性好,无需知道两端的碱基序列,信息量大,已在植物的遗传作图、种质鉴定、基因定位、遗传多样性等研究方面得到应用.本文对ISSR技术的原理和特点及其在作物遗传育种领域的应用进行了综述.
Inter-simple sequence repeat markers have emerged as an alternative system with reliability and advantages of microsatellites(SSR).ISSR primer can be designed without the knowledge about microsatellite regions,and simple design.ISSR marker has good poly morphism and repetition and can provide more information.Its technique has been widely used in the studies of genetic mapping,germplasm identification,gene mapping,genetic diversity in plant.Reviev of the principle and characteristics of ISSR and its application in crop genetics and breeding in this paper.
出处
《内蒙古民族大学学报(自然科学版)》
2011年第6期682-684,共3页
Journal of Inner Mongolia Minzu University:Natural Sciences
关键词
ISSR
作物遗传育种
应用
ISSR
Crop genetics and breeding
Application