山黧豆胚胎发育过程中ODAP和一些大分子物质含量的变化

THE CHANGES OF ODAP AND SOME MACROMOLECULES CONTENT DURING THE DEVELOPMENT OF LATHYRUS SATIVUS SEED

应用微量分析方法检测了山黧豆胚胎发育过程中ODAP毒素含量和核酸、蛋白质、糖类等大分子物质的含量变化。结果表明:每粒种子的ODAP含量随着胚的发育而增加。每粒种子DNA量随着细胞的迅速分裂而增加,R、蛋白质、淀粉含量随着胚的发育而成倍地增加,当进入心形胚时这些物质的增加更为迅速。如以每克干重中的含量来表示,那么ODAP、DNA及可溶性糖含量则随胚的发育而下降,其它大分子物质含量在胚发育前期升高,进入心形胚时,这些物质达到最高峰;到鱼雷胚时,这些物质含量开始下降,直到胚基本分化完全时,降到最低点;只有酸性蛋白质含量一直保持增长。

The results of determination of the changes in ODAP toxin, nucleic acid, protein and sugar content during development of Lathyrus sativus seed by microanalysis, show that the ODAP content per seed increases accompaning with embryogenesis, and so the other macromolecular content. The DNA content per seed increases when the embryonic cells are rapidly dividing, especially the content of RNA, protein and starch is double during embryogenesis, and they increase even more quickly at the heart stage. If the content of DNA, ODAP and the soluble starch are determined by dry weight per gram, respectively, they present the declining tendency during the development: of the seed; while the contents of other macromolecules inerease only at the early stage, and arrive, to the utmost almost at the heart stage, then begin to decline at the torpedo stage and decline to minimum until the embryo defferentiate completely. Only the acidic protein keeps its increasing in all the time of embryo development.