光肩星天牛幼虫纤维素酶活性的变化趋势

Cellulase in Larvae of Asian Longhorn Beetle Anoplophora glabripennis

光肩星天牛（Anoplophora glabripennis）（ALB,Asian longhorn beetle）属鞘翅目、天牛科、沟胫天牛亚科、星天牛属,是我国重要林木蛀干害虫（萧刚柔,1992）。中国东部光肩星天牛分布区位于100°—127°E、21°—43°N（阎俊杰,1985）;该虫现已在美国伊利诺斯州的芝加哥市、纽约州的纽约市和长岛以及新泽西州,加拿大的多伦多和安大略建立低密度种群,奥地利。

Asian longhorn beetle larvae were fed on host plants and artificial diet to investigate the variation of cellulase, and the activity of exoglucanase and β-glucosidase were measured. The results showed that larvae fed on host tree under natural growth situation had a significant reduction in both endoglucanase and β-glucosidase activities. The activity of endoglucanase in the first and second instars was higher than the followed three instars, and the activities of β-glucosidase in the former three instars are higher than the followed two instars. This trend also occurred in the larvae fed on artificial diet. The activity of endoglucanase in the 10, 20 and 30 days of age larvae was higher than that in the 80, 100 and 120 days of age larvae, and the activities of β-glucosidase reached a peak in the 60 days of age larvae, higher than the 80, 100 and 120 days of age larvae. Therefore it is suggested that ecological control measures related to cellulase inhibitor should be carried out on the first two instars larvae. According to its biological characteristics, the best time for controlling through inhibiting the activity of cellulase would be from September to November.