猪血蛋白的复合酶水解工艺条件优化研究

Study on optimum hydrolysis conditions of porcine blood protein by compound enzyme

为了提高猪血蛋白水解率以获得更加丰富的小分子肽及游离氨基酸,文章通过复合酶的筛选及水解条件的单因素实验和响应面优化研究,得出水解猪血蛋白最佳复合酶组合为风味蛋白酶与胰酶,其最佳水解工艺条件为:水解温度50.88℃,初始pH为8.15,复合酶配比为0.96∶1,底物浓度6%,水解时间6h。水解度模型预测值42.91%,实测值43.13%,证明所得回归模型拟合较好,可以用来预测实验结果。

In order to get more small peptides and free amino acids from porcine blood,optimal compound enzyme was selected and then it’s hydrolysis conditions were optimized by response surface methodology.The results showed that the best combination of compound enzyme was flavourzyme and Pancreatin.The optimum conditions were that temperature 50.88 ℃,pH 8.15,the ratio of flavourzyme and Pancreatinwas 0.96∶1,substrate concentration 6%,hydrolysis time 6 h.The value of model prediction was 42.91% and the measured was 43.13%,the model can be used to predict the experimental results.