花椰菜未受精子房、花托、花序轴离体培养诱导成苗研究

Studies on Plant Regeneration Induced from Rachis Receptacle and Unfertilized Ovary of Cauliflower

以花椰菜的花托、花序轴和未受精子房为外植体 ,诱导出不定芽 ,并进行继代增殖培养 ,在继代增殖培养基上每隔 2 5～ 30天 ,不定芽可以增殖 5～ 6倍 ,在不加激素的 1/2MS培养基上 ,试管苗生根率达到 98%以上 ,移栽成活率达到 95%以上。经观察 ,花托和未受精子房再生植株是由外植体直接分化而来 ,不经过愈伤组织阶段 ,这样可避免不良变异 。

Receptacle,rachis and unfertilized ovary of cauliflower were as explant for inducing plant regeneration and subcultured.Subcultured shoots on Ms 199 medium could be increased to 5-6 times in every 25-30 days.Rooting rate reached more than 98% on 1/2 Ms medium without any hormone.The surviving rate of the plantets transfered into soil reached more than 95%.Throngh abservation,We found that the plantlets came directly from receptacle and unfertilized ovary explants without callus stage,thus can avoid bad variation and keep genetic stability of culture materials.