超高效液相色谱-紫外检测法快速同时测定烟丝中的多酚

Fast and Simultaneous Determination of Phenolic Compounds in Cut Tobacco with Ultra Performance Liquid Chromatography Photodiode Array

建立了超高效液相色谱(UPLC)-紫外检测法同时快速测定烟丝中的绿原酸、芸香苷和莨菪亭。采用1%的乙酸水溶液对烟丝进行超声提取后,经0.2μm滤膜过滤直接进行UPLC分析。利用PDA检测器分别对绿原酸、芸香苷和莨菪亭进行定性定量。相对标准偏差为2.57%～4.57%,回收率为87.29%～91.67%。该法具有前处理简便、分析时间短、重现性好等优点,适合卷烟烟丝多酚类物质的批量快速检测。

A method based on Ultra Performance Liquid Chromatography Photodiode Array（UPLC-PDA） was developed for fast and simultaneous determination of scopoletin,chlorogenicacid and rutin in cut tobacco.The cut tobacco were extracted with 1% acetic acid under ultrasonic,the extract was detected by UPLC-PDA after filtered with 0.2 μm filter membrane.The results showed that the RSDs for the determination of polyphenols in cut tobacco were of 2.57%～4.57% with recoveries of 87.29%～91.67%.The method has advantages of easy to pretreat,low time consumption and high reproducibility,which is suitable for the fast analysis of phenolic compounds in cut tobacco.