摘要
目的研究云南省兰坪铅锌矿区矿石样品中的细菌群落结构。方法构建细菌16S rRNA高变区基因文库。结果矿石内部细菌种类丰富多样,文库测序结果表明矿石样品中细菌分属于4个门,27个属;其中变形菌门(Proteobacteria)类群是优势种群,占所有细菌总克隆数的51.7%,而且大多数属于γ-变形菌纲亚群。各克隆序列分析结果表明,其中部分细菌序列与已报道物种的相似性较低。结论表明云南省兰坪铅锌矿矿石样品中可能存在新的细菌种类。
Objective To study the bacterial community structure and diversity in ore samples from Lanping Zinc-lead Mine in Yunnan province.Method 16S rRNA gene library was constructed.Result A diverse bacterial community inhabits in the ore samples was revealed.Sequence analysis results showed that the library contained 4 bacterial phyla including 27 genera.Phylogenetic tree was constructed to confirm these results.The most abundant phylum was Proteobacteria,which accounted for 51.7% of all the obtained clones,and the most abundant subgroup within Proteobacteria was γ-Proteobacteria.NCBI BLAST analysis showed that some clones exhibited low levels of DNA similarity with any of the known 16S rDNA sequences in the database.Conclusion Some new species may exit in the ore.
出处
《中国微生态学杂志》
CAS
CSCD
2012年第2期109-112,共4页
Chinese Journal of Microecology
基金
云南省自然科学基金(KKSA200826046)
云南省后备人才基金(2009CI027)
