5年生人参出苗期几种脱氢酶活力比较

Comparison of Dehydrogenase Activity in 5-year Ginseng during Seedling Stage

目的对5年生人参出苗期参根和参苗中苹果酸脱氢酶(malate dehydrogenase,MDH)、乳酸脱氢酶(lactatedehydrogenase,LDH)、乙醇脱氢酶(Alcohol dehydrogenase,ADH)、葡萄糖-6-磷酸脱氢酶(glucose-6-phosphatedehydrogenase,G6PDH)、α-磷酸甘油脱氢酶(α-glycerophosphate dehydrogenase,α-GPD)5种脱氢酶的活力进行比较。方法采用中性缓冲液提取粗酶液,应用分光光度法测定MDH,LDH,ADH,G6PDH,α-GPD的活力。结果在人参出苗期,参根和参苗中的5种脱氢酶活力变化趋势有所不同,并在不同时期出现峰值。结论 MDH,LDH,ADH,G6PDH,α-GPD的活力可以作为人参生长过程中长势优劣的评价指标。

OBJECTIVE To compare the activities of malate dehydrogenase （MDH）, lactate dehydrogenase （LDH）, alcohol dehydrogenase （ADH）, glucose-6-phosphate dehydrogenase （G6PDH） and a-Glycerophosphate dehydrogenase （a-GPD） in 5-year Ginseng Radix in seedling stage. METHODS Adopt neutral buffer solution to extract the coarse enzyme. Use spectrophotometry to test the activities of MDH, LDH, ADH, G6PDH, α-GPD. RESULTS In seedling stage of Ginseng Radix, there were different changes trends of these five dehydrogenase in root and sprout. And there were peak values in different stages CONCLUSION The activities of MDH, LDH, ADH, G6PDH, α-GPD can be used as the evaluation indicators of quality of Ginseng Radix.