山西道地药材远志的毛细管电泳指纹图谱研究

Fingerprint Chromatogram of Polygalae Radix from Shanxi Province with HPCE

目的建立山西道地药材远志的毛细管电泳指纹图谱(CEFP),获得对照图谱,并与不同产地远志药材指纹图谱相比较,以控制远志质量。方法未涂层毛细管柱(75μm×57 cm,有效长度50 cm);缓冲液:25%甲醇的20 mmol.L 1硼砂溶液;分离电压:18 kV;检测波长:210 nm;温度:25℃;进样条件:0.5 psi,5 s。使用指纹图谱相似度评价软件进行数据处理,比较分析远志HPCE指纹图谱的相似度。结果建立了山西道地远志药材的HPCE指纹图谱共有模式,并比较了不同产地远志药材HPCE指纹图谱的相似度。结论所建立的CEFP具有较好的重复性,为远志药材质量控制提供了新方法。

OBJECTIVE To establish the high performance capillary electrophoresis （HPCE） fingerprint chromatogram of genuine Polygalae Radix from Shanxi and get the control chromatogram. METHODS The separation was carried out in an uncoated fused silica capillary （75 μm×57 cm, effective length 50 cm）. The running voltage was 18 kV. The buffer was 20 mmol-L-1 Na2B4O7 containing 25% methanol, and the wavelength was 210 nm. Data and chromatogram were dealt with ＂Computer Aided Similarity Evaluation＂ software in order to analyse the similarity of fingerprint chromatogram of Polygalae Radix from different districts. RESULTS The control fingerprint of HPCE of Polygalae Radix was established, and the similarity of fingerprint chromatogram of Polygalae Radix from different districts were compared. CONCLUSION This method is proved to be simple, rapid and accurate, and can be used as a new method for the quality control of Polygalae Radix.