低温驯化过程中油菜叶片钙、镁、铁的亚细胞分布特征(英文)

Subcellular Distribution of Calcium, Magnesium and Iron in Brassica napus Leaves during Cold Acclimatization

[目的]本文的目的是揭示钙、镁、铁等必需营养元素在油菜耐寒性中的作用。[方法]通过田间试验比较了不同环境条件下,中双11号(ZS)和甘油杂1号(GY)油菜(Brassica napusL.)叶片中钙、镁、铁浓度在亚细胞分布上的变化。[结果]油菜根系中钙、镁、铁浓度在低温驯化过程中显著升高,在地上部钙和镁浓度显著降低,而铁浓度显著升高。在叶片中,可溶态钙浓度显著升高,而细胞器和细胞壁结合态钙浓度显著降低;可溶态、细胞器和细胞壁结合态镁浓度均显著降低;而可溶态、细胞器和细胞壁结合态铁浓度均显著升高。同时,ZS的这些测定指标在两个环境条件下的变化幅度均大于GY,可能是因为GY是耐寒品种,而ZS不是。[结论]这些结果说明,提高地上部钙、镁、铁含量可能有助于提高油菜的耐寒性。

[Objective] The aim of the present study was to investigate the characteristics of calcium （Ca）, magnesium （Mg） and iron （Fe） subcellular distributions in oilseed rape （Brassica napus L.） leaves during cold acclimatization. [Method] A field experiment was conducted at two contrasting environments with three replicates and included a freezing sensitive variety （cv. Zhongshuang No. 11, ZS） and a freezing tolerant variety （cv. Ganyouza No. 1, GY）. [Result] The concentrations of Ca, Mg and Fe were significantly increased in plants roots, and the concentrations of Ca and Mg were significantly decreased but the Fe concentration was significantly increased in plants shoots. In leaves, the Ca concentration stored in soluble fraction was significantly increased and the Ca concentration of organelle-contained fraction and cell wall-associated fraction were significantly decreased with experiment time. The Mg concentrations stored in soluble fraction, organelle-contained fraction and cell wall-associated fraction were decreased with time. In contrary, the Fe concentration stored in soluble fraction, organelle-contained fraction and cell wall associated-fraction was significantly increased with time. Meanwhile, the changes of these measures of ZS were much higher than those of GY during cold acclimatization, which might be due to the GY is a freezing tolerant variety but ZS is not. [Conclusion] These results suggested that increasing the concentrations of Ca, Mg and Fe in plants shoots might increase the cold resistance of oilseed rape.