混合喂养法诱导营养性肥胖大鼠模型的实验研究

A Study of the Mixed Feeding Method in Establishing Diet Induced Obesity Rat Model

目的探讨用混合喂养法诱导营养性肥胖大鼠模型。方法 72只雄性幼年健康SD大鼠按照体质量随机分为对照组(基础饲料喂养,n=36)和模型组(高脂饲料+普通饲料混合喂养,n=36),分笼饲养,每周记录动物体质量、体长。以普通饲料组Lee.s指数〔(体质量g)1/3×103/体长cm〕正常值上限(均数±标准差)为肥胖确定标准,大于此上限为肥胖。结果造模第四周末,模型组大鼠体质量明显大于对照组,Lee.s指数、腹腔脂肪重量及脂肪系数也大于对照组,同时,模型组大鼠血浆三酰甘油及高密度脂蛋白、极低密度脂蛋白水平均低于对照组,但血糖、胆固醇及低密度脂蛋白水平显著高于对照组。造模第八周末,模型组体质量更明显增大,上述其他各项指标的异常进一步加重。结论混合喂养法是建立营养性肥胖大鼠模型的有效方法。

Objective To study the mixed feeding method in establishing diet induced obesity rat model （DIO）. Methods 72 male juvenile healthy SD rats were housed individually and divided into two groups randomly according to their body weight, control group （fed with CHOW, n =36）, and model group （mixed feeding method, fed with high fat diet and CHOW together, n =36）. Body weight （BW） and body length （BL） were measured weekly. The uper border of lee. Sindex [BW （g） 1/3 × 10^3/BL （cm） ] of control group was taken as criteria for obesity, individuals with values higher than that were deft- nited as DIO. Results Modeling the fourth week, rats body weight was significantly greater than the control group, Lee. s in- dex, abdominal fat weight and fat coefficient was also larger than the control group, while serum TG, HDL - C and VLDL - C were significantly higher, GLU, T- CHO, LDL - C showed a decreasing trend. Modeling the eighth week, the model group significantly increased body weight was more, the other indicators of abnormal further. Conclusions Mixed feeding method is effective for modelling DIO in rats.