酪酸梭状芽孢杆菌低成本培养基的优化

Determination of an economical medium for growth of Clostridium butyricum fermentum

本文重点在于提高酪酸梭状芽孢杆菌活菌数量，降低发酵培养基成本。通过对发酵培养基中不同碳源、氮源、生长因子等进行单因素研究，得到最佳培养基组成：可溶性淀粉10/L，豆粕（中性蛋白酶水解3h）20g／L，玉米浆3g／L。用此培养基在37℃培养24h，采用高层半固体琼脂试管法对酪酸梭状芽孢杆菌进行活菌计数，活菌数可达8．2×10^8cfu／mL.培养基中添加K2HPO45g／L、MgSO4·7H2O0．2g／L、MnSO3·H2O0．2g/L培养32h时，酪酸梭状芽孢杆菌芽孢转化率可达95％。

This artide focuses on improving the viable number of Clostridium butyricum and reducing the cost of fermentation medium. The optimal fermentation medium for Clostridium butyricum were obtained by the single factor test of carbon source, nitrogen source and growth factors design, with the composition of soluble starch 10 g/L, soybean meal（hydrolyzed for 3 h by neutral protease） 20 g/L, and corn steep liquor 3 g/L. After incubating under this condition for 24 h, the number of viable cells could get to 8.2 10^8 efu/mL by the high layer semi-solid agar tube method. In order to increase the spore forming rate, some inorganic elements and microelements should be added into the fermentation medium. In this way, the highest spore forming rate reached 95 % after fermentation for 32 h.