褐藻寡糖对小鼠低温力竭游泳运动的影响及机制

Effect and mechanisms of brown alga oligosaccharides on exhaustive exercise of mouse in low temperature

目的:探讨褐藻寡糖对小鼠低温力竭游泳运动的影响及机制。方法:将小鼠随机分成5组,空白组,对照组,3个实验组,空白组和对照组用生理盐水灌胃,3个实验组分别用0.75%、1.5%、3%不同浓度的褐藻寡糖进行灌胃,每只每天灌胃量0.3～0.5 mL,连续灌胃4周。之后对照组和试验组每组取小鼠7只,分别在(5±1)℃环境条件下进行无负重力竭游泳运动试验,观察并记录游泳力竭时间及行为改变。待小鼠死亡后(空白组断头处死),分别取出甲状腺、肾上腺、胰腺、胸腺和脾脏,用电子天平称重,并计算其系数。系数=腺体(mg)/体重(g)×100%。结果:褐藻寡糖能提高小鼠低温力竭游泳运动时间,1.5%褐藻寡糖组较对照组差异显著(P<0.05);甲状腺、肾上腺、胰腺、胸腺和脾脏系数增大。结论:褐藻寡糖能够提高小鼠的力竭游泳运动能力,1.5%浓度为最佳;一些内分泌腺和脏器不同程度参与了小鼠低温力竭游泳运动应激反应。

To study effects and mechanisms of brown alga oligosaccharides on exhaustive swim exercise of mouse. METHODS ： Mice were divided into 5 groups in random （n= 10）. The blank and control groups were affused into stomach with NaCI of 0. 9% and test groups were affused into stomach respectively with brown alginate oligosaccharide of 0. 75%, 1.5% and 3%. Affusing capacity was 0. 3 ~ 0. 5 mL per mouse per day and lasted for 4 weeks. Then, control and test groups were swum exhaustive on the conditions of （5 ＋ 1 ） ~C respectively, and observed the survival time and behavioral alteration. After death, thyroid, adrenal gland, pancreas, thymus and spleen were took out and weighted by electric scale and calculated their coefficients, coefficient = gland （mg）/weight （g） ~ 100%. RESULTS： Under the condition of low temperature, the time of exhaustive swim exercise of mice was prolong, and there was difference between 1.5% brown alga oligosaccharides group and control group, the difference was significant （P〈0. 05）. The coefficient of thyroid, adrenal glan, pancreas, thymus and spleen increased. CONCLUSION ： Brown alginate oligosaccharides can improve the capacity of exhaustive swim exercise of mouse, and the concentration of alginate oligosaccharides on 1.5 percent is the best. The endocrine participates stress activities of exhaustive swim exercise to different extents.