多发性硬化患者血清及脑脊液中干扰素-γ水平的研究

The changes of the serum and CSF levels of INF-γ in Multiple Sclerosis

目的研究多发性硬化患者血清及CSF中干扰素-γ水平的变化,探讨干扰素-γ在MS发病中的作用。方法选择58例河北医科大学第二医院住院MS患者,临床表现符合Poser等制定标准的确诊(definite)或很可能(probable)MS诊断。选择40例无血缘关系的健康人,年龄、性别均与病例组匹配,近期无感染、未应用免疫抑制剂。血液及CSF标本应用双抗体夹心酶联免疫(ELISA)方法测定干扰素-γ含量。结果急性期MS组血清及脑脊液IFN-γ水平较正常对照组及缓解期MS组明显升高,且脑脊液INF-γ增高程度明显。结论 INF-γ水平在MS急性期增高,并随病情的缓解而逐渐恢复正常,说明INF-γ在MS发病过程中起促进作用。

Objective To investigate the changes of the serum and CSF levels of INF-γ in MS,to define a possible pathogenic role of INF-γ during the progression of MS.Methods A case-control research was performed on 58 MS patients and 40 healthy individuals as control group.All patients fulfilled the criteria of MS proposed by Poser（1983）.Levels of INF-γ in test and control subjects were determined by means of sandwich enzyme-linked immunosorbent assay（ELISA）,Results The serum and CSF levels of INF-γ was found significantly increased in the acute-couse MS group compared with the healthy control group and the remitting-couse MS group.Conclusion The level of INF-γ increases in the patients with acute-couse MS,but not in the patients with remitting-couse MS.INF-γ may play an important role in the development of MS.