阿德福韦酯长期治疗对慢性乙型肝炎患者PBMCs中线粒体DNA的影响

The changes of mitochondrial DNA levels in peripheral blood monouclear cells in patients with chronic hepatitis b receiving long-term adefovir treatment

目的探讨长期服用阿德福韦酯对CHB患者外周血单个核细胞(PBMCs)线粒体的可能损伤。方法本研究包括18例服用阿德福韦酯2年、25例服用3年和22例未服用阿德福韦酯的CHB患者;采用实时定量PCR法检测各组患者PBMCs中线粒体DNA(mtDNA)含量(mtDNA/nDMA);采用ELISA法检测血浆丙二醛(MDA)、F2-isoprostanes含量;采用分光光度法检测血浆总抗氧化能力(TAOC)。结果 2年组患者mtDNA含量为0.6±0.4copies/cell,3年组为0.8±0.5copies/cell,均显著低于对照组(1.4±1.2 copies/cell,P均<0.05);2年组F2-iso-prostanes含量为1.5±0.8ng/ml,3年组为2.2±1.3ng/ml,显著低于对照组(3.7±2.7ng/ml,P均<0.05);2年组TAOC含量为3.0±1.1单位/毫升,3年组为2.3±1.4单位/毫升,显著低于对照组(4.3±1.8单位/毫升,P均<0.05)。3组间MDA含量无统计学差异(F=2.404,P>0.05)。结论长期应用阿德福韦酯治疗慢性乙型肝炎,对PBMCs中mtDNA水平有一定的影响,其意义还有待探讨。

Objective To observe the changes of mitochondrial DNA levels in peripheral blood monouclear cells in patients with chronic hepatitis B receiving long-term adefovir treatment.Methods Patients with CHB were divided into three groups：（1） 2 years group（n=18）：receiving adefovir for 2 years;（2）3 years group （n=25）：adefovir for 3 years;and （3） control group（n=22）：naive patients without antiviral treatment.The mtDNA of PBMCs were detected by quantitative real-time PCR. Plasma MDA and F2-isoprostanes were measured by ELISA and TAOC were detected by spectrophotometry.Results The mtDNA in PBMCs in the 2-year treatment group was 0.6＋0.4 copies/cell and in the 3-year treatment group was 0.8-＋0.5 copies/cell,both signifieantly lower than in the control group （1.4±1.2copies/cell,P〈0.05）;thc F2-isoprostanes in 2-year treatment group was 1.5±0.8 ng/ml and in 3-year treatment group was 2.2±1.3ng/ml,much lower than that in the control group（3.7±2.7 ng/ml,P〈0.05）;the TAOC in 2-year treatment group was 3.0±1.1 IU/ml,and in 3-year treatment group was 2.3±l.4IU/ml, significantly lower than in the control group （4.3±1.8 IU/ml,P〈0.05）.Conclusion The long-term administration of adefovir might injure mitochondrial DNA.