转Cry1Ac/CpTI栽培稻外源基因渗入普通野生稻中可稳定的遗传和表达

Exogenous Cry1Ac/CpTI of Commom Wild Rice by Introgressing from Transgenic Cultivated Rice can be Stable Inheritance and Expression

转基因水稻的研发以及环境释放,外源基因通过花粉逃逸到野生近缘种中,可能带来的生态效应愈来愈受到人们的关注。本研究采用人工杂交技术将Cry1Ac/CpTI双价抗虫基因从转基因栽培稻渗入普通野生稻(Oryza rufipogon Griff.)中,获得普通野生稻F1及分离后代F2、F3、F4、F5和回交后代BC1F1和BC1F2,并对渗入外源基因的普通野生稻中的外源基因的遗传和表达进行了分析,试图阐明转基因栽培水稻外源基因的逃逸,可能在近缘野生栽培稻中稳定遗传的事实。研究结果显示:在野生栽培稻中外源基因的拷贝数与转基因的供体栽培稻完全一致。外源基因在普通野生稻中传代过程中插入位点的遗传也是稳定的。Cry1Ac杀虫蛋白在野生稻的不同世代中的表达模式和表达量与其转基因的供体栽培稻大致一致。本研究说明,转基因水稻外源基因一旦逃逸,有可能渗入近缘野生稻中,并在近缘野生稻中可稳定的遗传与表达,但转基因水稻外源基因逃逸是否对其近缘物种造成非预期生态效应还需要进一步对其进行生态适合度评价。

With the rapidly development of transgenic rice and increasing environmental release,escape of foreign genes through pollen into wild relatives happens possible,the potential ecological effects may become more and more attention.In this study,Cry1Ac/CpTI bivalent transgenic insect-resistant genes from cultivated rice introgressing into common wild rice（Oryza rufipogon Griff.） were carried out to generate F1 and their offspring of F2,F3,F4 and F5,as well as backcross progenies,BC1F1 and BC1F2,and also inheritance and expression of foreign genes in wild rice offspring were analyzed in order to clarify whether or not escape of foreign genes from the transgenic cultivated rice might be stable inheritance and expression in the wild relatives of rice.The results showed that exogenous gene copy number in the wild rice offspring was completely identical to the gene donor of cultivated rice.The insertion loci of foreign genes in common wild rice were also quite stable.The expression patterns and expression levels of insecticidal protein Cry1Ac in the different generations of wild rice were almost consistent to that of their of gene donor of cultivated rice.This study illustrated that foreign genes of transgenic rice once escaping into wild relatives might be introgressing into other rice cultivar and wild relatives of rice,The foreign genes can be stable inherited and expressed,However,whether or not might foreign gene escape from transgenic rice impact on its closely relative species to cause its non-expected ecological effects,which needs further assessment for its ecological fitness.