利用分子标记辅助选择将抗稻瘟病基因Pi-9(t)渗入水稻恢复系泸恢17

Introgressing Blast Resistant Gene Pi-9(t) into Elite Rice Restorer Luhui17 by Marker-Assisted Selection

本研究采用杂交和分子标记辅助选择技术,将亲本材料P2的水稻广谱高抗稻瘟病基因Pi-9(t)导入杂交水稻恢复系泸恢17,再利用抗稻瘟病基因Pi-9(t)的特异分子标记pB8检测该目的基因,获得68份携有Pi-9(t)基因型的回交株系。基因型鉴定表明株系WR1023、WR1043、WR1056和WR1062为均含有Pi-9(t)基因纯合的回交后代株系。表型分析表明WR1023和WR1056株系农业性状已稳定,且其株叶型、抗稻瘟病及配合力较好,我们认为可以用作育种抗性亲本材料。

In this research,we introgressed the broad-spectrum blast resistant gene Pi-9（t） from the donor parent P2 into hybrid restorer Luhui17 by using backcross approach and molecular marker-assisted selection（MAS） technique.We detected the target gene through the specific molecular marker pB8 that close linked tothe blast resistant gene Pi-9（t）.As a result,sixty eight lines carrying broad-spectrum blast resistant gene Pi-9（t） were obtained,and then four lines,WR1023,WR1043,WR1056 and WR1062,were identified to be homozygous backcross lines with resistant gene Pi-9（t） by molecular phenotyping identification.Furthermore,the lines,WR1023 and WR1056 lines,were proved to have genetically stable agronomic characteristics,that demonstrated good plant architecture,high blast resistance and stable restoring ability,Therefore we suggested that these lines should be used as resistant gernplasms in in rice hybrid breeding program.