响应面法优化超声波提取玉米醇溶蛋白工艺

Optimization of ultrasonic extraction of zein from corn gluten meal by response surface methodology

利用响应面法对玉米黄粉中醇溶蛋白的提取工艺进行优化。根据中心组合试验设计原理采用4因素5水平响应面分析法,对各个因素的显著性和交互作用进行分析。结果表明:玉米醇溶蛋白超声提取的最佳条件为超声时间27 min、超声功率480 W、乙醇体积分数70%、液料比10:1(mL/g),该条件下玉米醇溶蛋白提取率可达70.71%,纯度为90.33%。

Response surface methodology was used to optimize the extraction processing of zein from corn gluten meal. Based on the analysis of significance of four factors and cross-interaction among factors, the results showed that the optimal extraction conditions were as follows： ultrasonic treatment time was 27 min, ultrasonic power was 480 W and alcohol concentration was 70% with the ratio of liquid to material 10：1（mL/g）. Under the optimal extraction conditions, the extraction rate of zein was up to 70.71% and the purity was 90.33%.