高效液相色谱外标法测定食用油脂中的叔丁基对苯二酚(TBHQ)

Determination of tert-butyl-hydroquinon in edible oil by HPLC with external standard method

建立了高效液相色谱(HPLC)外标法测定食用油脂中叔丁基对苯二酚(TBHQ)含量的方法。采用C18反相色谱柱(4.6 mm×15 cm,5μm)色谱柱,以乙腈:水=90:10(V/V)作为流动相:流速:0.8 mL/min;检测波长:292 nm。所测TBHQ在5～ 200 mg/kg之间具有较好的线性相关性,平均回收率为101.46%。结果显示,本法操作简便,重现性好,结果准确。

A high performance liquid chromatogrphy with external standard method for the determination of tert-butyl-hydroquinon in edible oil was established. The chromatographic separation was achieved with a mixture of methanol and water（V：V=90：10） as mobile phase on Alltima C18 column（250 mmx4.6 mm, 5 tim）. The flow rate as 0.8 mL/min and 292 nm was set as determination wave length. The obtained results indicated that the developed method is simple, accurate and reliable for the determination of tert-butyl-hydroquinon in edible oil with a good linearity within 5-200 mg/kg and the recovery ranges of 101.46%.