RP-HPLC法同时测定紫苏子中α-亚麻酸和亚油酸的含量

RP-HPLC simultaneous determination of α-linolenic acid and linoleic acid in Fructus Perillae

目的:建立RP-HPLC法同时测定紫苏子中α-亚麻酸和亚油酸的含量。方法:色谱柱为Agilent Eclipse XDB-C18(150 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-水(90∶10),流速1 mL·min-1,柱温30℃,检测波长205 nm。结果:α-亚麻酸、亚油酸浓度分别在14.45～144.5μg·mL-1(r=0.9998)和5.75～57.5μg·mL-1(r=0.9999)范围内与峰面积呈良好的线性关系,平均回收率(n=6)分别为100.2%(RSD=2.3%)和101.2%(RSD=1.5%)。结论:本法简便、快速、准确,适用于紫苏子及紫苏子油中α-亚麻酸和亚油酸的同时测定。

Objective： To establish an RP - HPLC method for simultaneous determination of a - linolenic acid and linoleic acid in Fructus Perillae. Methods：The separation was carried out on an Agilent Eclipse XDB -C18 （150 mm ×4. 6 mm,5 μm） column with a mixture of acetonitrile -water （90： 10） as the mobile phase at the flow rate of 1.0 mL· min-1 ;The column temperature was maintained at 30℃ and the detective wavelength was set at 205 nm. Results：The calibration curves of a - linolenic acid and linoleic acid were linear in the ranges of 14.45 - 144. 5μg · mL- 1 （ r =0. 9998 ） and 5.75 - 57.5 μg · mL - 1 （ r = 0. 9999 ）, respectively. The average recoveries （ n = 6 ） were 100. 2% （RSD = 2.3% ） and 101.2% （ RSD = 1.5% ）. Conclusion： The method is simple, rapid and accurate, which is suitable for simultaneous determination of a - linolenic acid and linoleic acid in Fructus Perillae and Perilla oil