Ca^(2+)螯合法纯化花生壳黄酮工艺研究

Study on purification of flavonoids from peanut shells based on Ca^(2+) chelating technology

基于黄酮化合物与金属离子的螯合作用,以花生壳黄酮及木犀草素的含量为指标,研究了花生壳黄酮的纯化方法。结果表明,用Ca2+纯化花生壳黄酮的最优条件为黄酮粗提物溶液pH为9.0,CaCl2:黄酮粗提物质量比为1:10,黄酮粗提物初始浓度为10.0mg/mL。最佳条件下黄酮的含量从初始的11.0%提高到27.84%。经高效液相色谱分析,结果表明纯化前黄酮粗提物中木犀草素的含量为0.87%,经Ca2+螯合纯化后,木犀草素的含量提高到1.97%。

Using flavonoids of peanut shells and the content of Luteolin as the key parameters,the purification of flavonoids was studied based on the chelating activity of flavonoids with metal ions.The results indicated the optimal purification conditions：the pH of flavonoids extracts solution was 9.0,calcium chloride flavonoids extracts ratio（w/w） was 1：10 and initial concentration of flavonoids extracts was 10.0mg/mL.Under the optimum condition,the content of flavonoids was up to 27.84% from the initial 11.0%.The Luteolin content before and after the purification were determined by HPLC and the analysis revealed that the content of Luteolin increased from 0.87% to 1.97%.