沙棘口服液中5-羟色胺的电化学检测

Electrochemical determination of serotonin in oral liquid of fructus hippophae

采用电化学沉积法制备咖啡酸膜修饰电极,研究了5-羟色胺(5-HT)在该修饰电极上的电化学行为。结果表明,高浓度的抗坏血酸(AA)和肾上腺素(EP)共存不干扰5-HT的检测,5-HT在0.41 V和-0.12 V出现一对灵敏的氧化还原峰,其氧化峰电流与浓度在2.0×10-6～6.0×10-5mol/L范围内呈良好的线性关系,检测下限为8.0×10-7mol/L。该修饰电极制备简单,可准确检测神兴沙棘欣之安口服液中5-HT的含量。

Caffeic acid-modified electrode was constructed by potentiostatic technique.The electrochemical behavior of serotonin（5-HT） at modified electrode has been investigated by cyclic voltammetry（CV）.The proposed electrode exhibits a promotion effect on the oxidation of 5-HT,and the interferences of ascorbic acid and epinephrine for determination of 5-HT have been investigated.The oxidative peak current obtained（ipa） is linearly dependent on the 5-HT concentrations in the range of 2.0×10-6~6.0×10-5 mol/L with the detection limit of 8.0×10-7 mol/L.This sensor was further applied for the determination of 5-HT in Chinese traditional drug Oral Liquid of Fructus Hippophae.