摘要
建立了含乳饮料中苯甲酸、山梨酸钾等添加剂的高效液相色谱仪同时检测的方法。含乳饮料经前处理后,由C18反相色谱柱进行分离,流动相A：磷酸盐缓冲溶液（1.25 g K2HPO4与1.25 gKH2PO4加水定容至500 mL）,流动相B：乙腈,流动相A、B按9∶1比例洗脱,流速0.6 mL/min,进样量为10μL。苯甲酸检测波长为225 nm,山梨酸钾的检测波长为240 nm,水杨酸的检测波长为225 nm。该方法线性范围为0.6~30μg/mL,检测限为0.06μg/mL。
A HPLC method for the determination of benzoic acid,potassium sorbate,salicylic acid in milk beverage was established and reported.The sample was extracted and the components were separated on a C18 reversed phase column eluated with a mobile shase of Acn/water（1.25 g K2HPO4 and 1.25 g KH2PO4 dissolved in 500 mL water）,and detected by the DAD detector.Linearity of the calibration curve was in the range of 0.6~30.0 μg/mL,and the detection limit was 0.06 μg/mL.The method was already applied to real sample analysis.
出处
《化学世界》
CAS
CSCD
北大核心
2012年第1期24-25,30,共3页
Chemical World
基金
上海市科学技术委员会资助(09142200901
101422011000)
