摘要
以荧光微球作为新型标记物,采用EDC法进行偶联,制备莱克多巴胺抗体荧光微球复合物,复合物粒度均一、性质稳定,并以之为探针建立莱克多巴胺荧光微球免疫层析检测方法,检测限为2.5ng/mL,且特异性强、检测范围宽。与胶体金免疫层析方法相比,荧光微球免疫层析方法的灵敏度更高,新型的荧光微球可以提高免疫层析方法的灵敏度。
A composition,with a stable character and uniform size,and which contains fluorescent microspheres as a novel label,was obtained successfully by a EDC one-step method.A fluorescent lateral flow assay for detecting ractopamine which has high sensitivity（LOD available is 2.5 ng/mL）,satisfying specificity and a wide range for detecting was developed based on this composition as probe.The method of fluorescent microspheres lateral flow assay is more sensitive than that of colloidal gold lateral flow assay.The novel fluorescent microspheres do contribute to improve the sensitivity of immunochromatographic assay.
出处
《食品与机械》
CSCD
北大核心
2012年第1期73-77,共5页
Food and Machinery
基金
国家自然科学基金项目(编号:30960301)
江西省教育厅科学技术研究项目(编号:GJJ09284)
关键词
荧光微球
莱克多巴胺
免疫层析
检测
fluorescent microspheres
ractopamine
immunochromatography
detection
