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siRNA技术在哮喘治疗中的应用 被引量：3

siRNA as a therapy for asthma treatment
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摘要 RNA干扰是由双链RNA引发的序列特异的基因沉默现象,其中长度为21～23nt的小RNA分子(small inter-fering RNA,siRNA)是引起RNA干扰现象的直接原因。利用siRNA使基因沉寂从而调节目的蛋白的表达,在疾病的基因治疗中有巨大的应用价值。哮喘是一种常见的多发病,在治疗上仍面临极大的挑战。利用siRNA技术治疗哮喘是当今最新的一种治疗手段,虽然应用才刚刚起步,但其革命性的治疗理念,无疑给哮喘患者带来了新的希望。 RNA interference is the process of gene silencing by 21-23 nucleotides double-stranded small interfering RNA molecules. RNAi via siRNAs now demonstrates great value in gene therapy by interfering with the expression of a specific gene. Asthma as a chronic airway inflammatory disease faces great challenges in treatment. However the therapeutic advantages of siRNAs show great promise of applying siRNAs to alleviate asthma symptoms.
作者 倪振华 王雄彪
机构地区 上海市普陀区中心医院实验中心 上海市中医药大学中西医结合肿瘤介入研究所 上海市普陀区中心医院呼吸科
出处 《中国当代医药》 2012年第2期12-13,共2页 China Modern Medicine
基金 上海市科委基础研究重点项目(09JC1412900) 上海市科委引领计划(10411969100) 上海市教委科研创新项目(10YZ54)
关键词 RNA干扰 SIRNA 哮喘 治疗 RNA interference siRNA Asthma Therapy
分类号 R562.25 [医药卫生—呼吸系统]
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参考文献20

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共引文献2

同被引文献40

  • 1姜山,石永兵,施晓松,谭锦泉.哮喘患者外周血CD4^+和CD25^+调节性T细胞的变化及意义[J].新乡医学院学报,2006,23(5):531-532. 被引量：23
  • 2董爱琴,徐泳.肺部吸入给药系统研究进展[J].江西医药,2007,42(1):82-85. 被引量：4
  • 3赵宝林,方向明.平喘宁对哮喘豚鼠白三烯B4、可溶性细胞间粘附分子-1含量的影响[J].中国中医药信息杂志,2007,14(4):35-37. 被引量：4
  • 4林少微,王雪华,郑高哲,陈淑贞.RNAi的研究进展[J].中国医药导报,2007,4(10Z):7-9. 被引量：3
  • 5Nauta A J, Engels F, Knippels LM, et al. Mechanisms of allergy and asthma[ J]. Eur J Pharmacol. 2008, 585 : 354 - 360.
  • 6Hammond SM, Bernstein E, Beach D, et al. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophi- la cells[J]. Nature. 2000, 404(6775): 293-296.
  • 7Zamore PD, Tuschl T, Sharp PA, et al. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals[ J]. Cell. 2000, 101 ( 1 ) : 25 - 33.
  • 8Huang HY, Lee CC, Chiang BL. Small interfering RNA against interleukin-5 decreases airway eosinophilia and hyper-responsive- ness[J]. GeneTher. 2008, 15(9): 660 -667.
  • 9Pfaffl MW, Horgan GW, Dempfle L. Relative expression soft- ware tool (REST) for group-wise comparison and statistical anal- ysis of relative expression results in real-time PCR [ J]. Nucleic Acids Res. 2002, 30(9) : e36.
  • 10Behlke MA. Progress towards in vivo use of siRNAs[ Jl. Mol T- her. 2006, 13(4) : 644 -670.

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中国当代医药
2012年 第2期

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