摘要
目的应用HPLC测定中国常见植物性食品中嘌呤的含量。方法采用Waters Atlantis T3柱(4.6mm×250mm×5μm),以10.0mmol/L甲酸铵(pH3.6)-甲醇(99∶1,V/V)为流动相,流速:1.0ml/min,柱温:30℃,检测波长:254nm。结果不同种类植物性食品中嘌呤含量有明显差别。干菌类和干豆类及制品中嘌呤含量普遍高于其他食品,蔬菜类及制品和水果类及制品中嘌呤含量普遍较低。总体上呈现出干菌藻类〉干豆类及制品〉鲜菌藻类〉坚果、种子类〉谷类及制品〉蔬菜类及制品〉薯类、淀粉及制品〉水果类及制品。结论植物性食品中干菌藻类和干豆类及制品中嘌呤含量较高,其他种类食品嘌呤含量较低,不同种类植物性食品中嘌呤含量差异较大。
Objective To determine the content of purine in plant food in China with HPLC.Method HPLC analysis was applied on Waters Atlantis T3 column(4.6mm×250mm×5μm),using 10.0 mmol/L NH4COOH(pH 3.6) and CH3OH(99%/1%) as mobile phase and running at a flow rate of 1.0 ml/min.The column temperature was 30℃,and the detection wavelength was at 254nm.Results The content of purine varied significantly in different kinds of plant food.The content of purine in dried fungi and dried legumes and legume products was higher than that in other food,the content of purine in vegetables and vegetable products and fruits and fruit products was low.As a whole,the content of purine was: dried fungi and algae dried legumes and legume products nuts and fresh seeds fungi and algae cereal and cereals products vegetables and vegetable products fruit and fruit products tubers,starches and products.Conclusion The content of purine of dried fungi and algae and dried legumes and legume products in plant food was high.The content of purine was varied significantly in different kinds of plant food.
出处
《卫生研究》
CAS
CSCD
北大核心
2012年第1期92-95,101,共5页
Journal of Hygiene Research
基金
达能膳食营养与宣教基金项目(No.DIC2008-002)