骨疏丹闪式提取工艺优化

Optimization of Extraction Technology of Gushudan by Smashing Extraction Method

目的:优化骨疏丹闪式提取工艺。方法:以骨疏丹中总黄酮、总香豆素含量,柚皮苷、朝藿定B、淫羊藿苷、蛇床子素和丹参酮ⅡA含量之和及浸膏率为评价指标,对骨疏丹的闪式提取工艺进行考察。结果:最佳提取工艺为12倍量75%乙醇,闪式提取3次,每次2 min。按最佳工艺所得总黄酮质量分数26.27 mg.g-1,总香豆素17.93 mg.g-1,柚皮苷、朝藿定B等5个成分含量之和9.213 mg.g-1,浸膏率23.50%。结论:优选出的闪式提取方法稳定性好、简便可行。

Objective：To optimize extraction technology of Gushudan by smashing extraction method.Method：Smashing extraction technology of Gushudan was studied by the content of total flavonoids and total coumarins,the total contents of naringin,epimedin B,icariin,osthole and tanshinoneⅡA,and extract ratio from Gushudan as indexes.Result：Optimum extraction technology was as follows：smashing extracted 3 times with 12 times the amount of 75% ethanol as dissolvent,2 minutes every time.The content of total flavonoids,total coumarins,total contents of naringin,epimedin B,icariin,osthole and tanshinoneⅡA,and extract ratio were 26.27,17.93,9.213 mg·g-1,and 23.50% respectively.Conclusion：Optimum smashing extraction technology was stable and easy.