粉葛提取物的制备工艺

Preparation Technology of Dry Extract from Pueraria thomsonii

目的:研究粉葛提取物的制备工艺。方法:以葛根素含量为考察指标,采用单因素及正交试验法筛选粉葛的提取工艺;以葛根素含量及特征图谱主要特征峰面积的转移率为考察指标,评价粉葛提取物的各工艺步骤。结果:优选的提取工艺为采用8倍量30%乙醇,加热回流3次,每次2 h,葛根素提取率达97%以上;主要制备工艺步骤为饮片→醇提液→浓缩液→醇沉液→浸膏→提取物,各工艺步骤的葛根素转移率分别为97.89%±2.17%,81.70%±2.12%,74.92%±1.92%,72.15%±1.06%,50.64%±2.57%(n=3),表明葛根素在干燥步骤中损失较大;特征图谱结果显示,各工艺步骤均能保留粉葛的7个特征峰,其中峰1及峰6的转移率较高,峰2,3,4,5,7的转移率均较低;粉葛提取物的得率5.09%±0.06%,葛根素质量分数(29.262±1.500)mg.g-1。结论:工艺合理、稳定、可行,为含粉葛的中药复方制剂工艺提供了参考。

Objective：To study on preparation technology of dry extract from Pueraria thomsonii.Method：Extraction technology of P.thomsonii was optimized by single factor and orthogonal experiment by taking the content of puerarin as index.Technology steps of dry extract from P.thomsonii was estimated by the content of puerarin and transfer rate of main feature peak area of specific chromatogram as index.Result：Optimum extraction technology was established as follows：heating reflux extracted 3 times with 8 times the amount of 30% ethanol for 2 h each time,extraction ratio of puerarin was more than 97%;Main preparation technology steps were：decoction pieces→alcohol extract fluid→concentrated solution→alcohol precipitating fluid→extract→dry extract,transfer ratio of puerarin in technology steps were 97.89%±2.17%,81.70%±2.12%,74.92%±1.92%,72.15%±1.06%,50.64%±2.57%（n=3）,it showed puerarin losed more in drying step;It showed in specific chromatogram that all technology steps remained 7 characteristic peaks of fingerprint,and transfer ratio of peak 1 and peak 6 was high,but it was low of peak 2,3,4,5,7;Yield of dry extract from P.thomsonii was 5.09%±0.06%,and the content of puerarin was（29.262±1.500） mg·g-1.Conclusion：This technology was simple,feasible and could provide a reference for technology of P.thomsonii compound preparation.