星点设计-效应面法优化小檗碱β-环糊精包合物制备工艺

Optimization of Preparation Technology of Berberine-β-Cyclodextrin Inclusion Complex by Central Composite Design and Response Surface Method

目的:优化小檗碱-β-环糊精包合物的制备工艺。方法:以小檗碱-β-环糊精包合物的包合率和收率为考察指标,采用星点设计考察包合温度、包合时间以及主客分子投料比对包合工艺的影响,对结果进行多元线性回归、二次多项式和三次多项式拟合,并根据最佳数学模型进行预测。结果:多元线性回归方程的复相关系数较低,三次多项式拟合结果优于二次多项式拟合结果,小檗碱-β-环糊精包合物的最优包合工艺为β-环糊精与小檗碱的投料质量比3.65,包合时间4.5 h,包合温度64℃,包合率和收率预测值与理论值偏差分别为3.28%,2.63%。结论:星点设计-效应面法优化小檗碱β-环糊精包合物制备工艺具有很好的预测性。

Objective：To optimize preparation process of berberine-β-cyclodextrin inclusion complex by central composite design and response surface method.Method：With inclusion ratio and yield of berberine-β-cyclodextrin inclusion complex as indexes,influence of inclusion temperature,inclusion time,and ratio of β-CD-berberine to inclusion process was investigated by response surface method,results were fitted by multi-linear equation,second-order and third-order polynomial equation,and optimal inclusion process was predicted according to formulation.Result：Multiple correlation coefficients from multi-linear equation were worse,and multiple correlation coefficients from third-order polynomial equation were prior to second order polynomial equation.Optimum inclusion technology of berberine-β-cyclodextrin inclusion complex were as follows：ratio of β-CD-berberine 3.65∶1,inclusion time 4.5 h,inclusion temperature 64 ℃.Bias of inclusion rate and yield between observed and predicted values were 3.28% and 2.63% respectively.Conclusion：Using central composite design and response surface methodology to optimize preparation of berberine-β-cyclodextrin inclusion complex had good prediction.