UPLC测定半夏中胡芦巴碱的含量

Detemination of Trigonelline in Pinellia ternate by UPLC

目的:采用超高效液相(UPLC)及亲水作用色谱法(HILIC,hydrophilic interaction chromatography)测定半夏中胡芦巴碱的含量,建立半夏药材质量评价方法。方法:半夏细粉经50%的甲醇超声提取后,采用ACQUITY UPLC BEH HILIC色谱柱(2.1 mm×100 mm,1.7μm)进行胡芦巴碱含量的测定,流动相为乙腈-10 mmol.L-1醋酸铵(80∶20),流速0.4 mL.min-1,检测波长265 nm,进样量10μL。结果:盐酸胡芦巴碱在1.125～72 nmol.mL-1线性关系良好(r=0.999 6,n=7),平均回收率为99.7%(RSD 1.4%)。结论:该方法专属性强、重复性好、稳定、可控,可作为半夏药材质量控制的方法。

Objective：To develop UPLC and a hydrophilic interaction chromatography（HILIC） method for the determination of trigonelline in Pinellia ternate.Method：After extracting trigonelline in the fine powder of Pinellia ternate by ultrasonic extraction with 50% methanol,the separation was performed at ACQUITY UPLC BEH HILIC column（2.1 mm × 100 mm,1.7 μm）.The mobile phase consisted of acetonitrile-10 mmol.L-1 ammonium acetate（80∶ 20）,and the flow rate was 0.4 mL.min-1.The detection wave length was set at 265 nm.The sampling volume was 10 μL.Result：The method has good linearity in the range of 1.125-72 nmol.mL-1 for trigonelline （r=0.999 6,n=7）.The average recovery was 99.7%（RSD 1.4%）.Conclusion：The method is specific,accurate and stable.It can be used for determination of trigonelline in Pinellia ternate.