摘要
目的:采用超高效液相(UPLC)及亲水作用色谱法(HILIC,hydrophilic interaction chromatography)测定半夏中胡芦巴碱的含量,建立半夏药材质量评价方法。方法:半夏细粉经50%的甲醇超声提取后,采用ACQUITY UPLC BEH HILIC色谱柱(2.1 mm×100 mm,1.7μm)进行胡芦巴碱含量的测定,流动相为乙腈-10 mmol.L-1醋酸铵(80∶20),流速0.4 mL.min-1,检测波长265 nm,进样量10μL。结果:盐酸胡芦巴碱在1.125~72 nmol.mL-1线性关系良好(r=0.999 6,n=7),平均回收率为99.7%(RSD 1.4%)。结论:该方法专属性强、重复性好、稳定、可控,可作为半夏药材质量控制的方法。
Objective:To develop UPLC and a hydrophilic interaction chromatography(HILIC) method for the determination of trigonelline in Pinellia ternate.Method:After extracting trigonelline in the fine powder of Pinellia ternate by ultrasonic extraction with 50% methanol,the separation was performed at ACQUITY UPLC BEH HILIC column(2.1 mm × 100 mm,1.7 μm).The mobile phase consisted of acetonitrile-10 mmol.L-1 ammonium acetate(80∶ 20),and the flow rate was 0.4 mL.min-1.The detection wave length was set at 265 nm.The sampling volume was 10 μL.Result:The method has good linearity in the range of 1.125-72 nmol.mL-1 for trigonelline (r=0.999 6,n=7).The average recovery was 99.7%(RSD 1.4%).Conclusion:The method is specific,accurate and stable.It can be used for determination of trigonelline in Pinellia ternate.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第4期85-87,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点基础研究发展计划("973计划")(2011CB505300-05)
关键词
亲水作用色谱
半夏
胡芦巴碱
HILIC method
Pinellia ternate
trigonelline