十大功劳HPLC指纹图谱及聚类分析

Study on HPLC Chromatographic Fingerprint of the Mahonia Fortunei

目的:对十大功劳的HPLC指纹图谱及聚类分析进行研究,用以评价与控制药材内在质量。方法:采用HPLC法分析阔叶、长柱十大功劳的指纹图谱,运用指纹图谱相似度评价和聚类分析进行研究。结果:建立了十大功劳指纹图谱,确定10个色谱峰为共有峰,方法的精密度、稳定性、重现性良好,其指纹图谱相似性高,特征性和专属性强。结论:阔叶、长柱十大功劳的指纹图谱化学成分基本相同,但含量差异较大,相似度与聚类分析显示二者无明显差异,利用指纹图谱可对十大功劳进行质量控制。

Objective：To establish the method of fingerprint analysis on Mahonia Forunei for controlling the quality.Method：The main components of the Mahonia Forunei from different sampes were determined by HPLC.Result：Ten common peaks were confirmed on the fingerprint,studying on the fingerprint of the Mahonia Fortunei.Conclusion：The method is reliable and accurate with good reproducibility and characteristic,providing a scientific base for controlling the quality of Mahonia Forunei by establishing fingerprint of the Mahonia Forunei.