西藏红雪茶多糖含量测定

Determination of Lethariella cladonioides Polysaccharide from Tibet

目的:研究西藏红雪茶多糖含量测定的方法。方法:采用苯酚-硫酸法测定总糖含量,3,5-二硝基水杨酸(DNS)法测定还原糖含量,两者的差值即为多糖含量。结果:总糖标准曲线方程为OD=0.051 4C-0.0146(r=0.999 2),还原糖标准曲线方程为OD=0.022 9C-0.086(r=0.999 9),两者分别在2.5～12.5,8～40 mg.L-1有良好的线性关系。测得LC,LCA,LCP多糖含量分别为63.6%,82.6%,57.6%。结论:该方法操作快速、简便、灵敏、准确、重复性好。

Objective：To establish a method for the determination of polysaccharide in Lethariella cladonioides.Method：Using microplate reader,the content of total sugar was determined by means of phenol-sulfuric acid method and 3,5-dinitro salicylic acid（DNS） method was used for the determination of reducing sugar,the difference between the two was the polysaccharide content.Results：The total sugar standard curve equation was OD=0.051 4 C-0.014 6（r=0.999 2） and reducing sugar standard curve equation was OD =0.022 9C-0.086（r=0.999 9）.Respectively the linear range was 2.5-12.5 mg.L-1 and 8-40 mg.L-1.Thepolysaccharide content of LC,LCA,LCP were 63.6%,82.6%,57.6%.Conclusion：The method is fast,simple,sensitive,accurate and reproducible.