不同地区白术药材及饮片中白术内酯Ⅱ的含量测定

Determination of Atractylenolide Ⅱ in Crude Drugs and Slices of Atractylodes macrocephala Koidz.from Different Areas by HPLC

目的:采用HPLC测定不同地区白术药材及饮片中白术内酯Ⅱ的含量。方法:采用Tigerkin C18(4.6 mm×200 mm,5μm)色谱柱,流动相甲醇-水(77∶23),流速1.0 mL.min-1,检测波长276 nm,柱温30℃。结果:测定了10个不同产地的白术药材和20个不同地区市售白术片及麸炒白术片中白术内酯Ⅱ的含量。结论:来源于不同地区的白术药材、白术片以及麸炒白术片中白术内酯Ⅱ的含量差别均较大。

Objective：To determine the content of atractylenolide Ⅱ in crude drugs and slices of Atractylodes macrocephala from different areas by HPLC.Method：The samples were separated on Tigerkin C18column（4.6 mm×200 mm,5 μm）with the mobile phase of methanol-water（77∶23）.Flow rate was 1.0 mL·min-1.The detection wavelength was set at 276 nm.Column temperature was maintained at 30 ℃.Result：The contents of atractylenolide Ⅱ in crude drugs from 10 places of origin and slices and processing slices of A.macrocephala from 20 regions was determined.Conclusion：The contents of atractylenolide Ⅱ in crude drugs and slices of A.macrocephala from different regions was different.