虎杖总蒽醌对糖尿病肾病模型小鼠肾皮质TGF-β_1,Smad3的影响

Influence of Polygonum Cuspidatum Anthraquinone on TGF-β_1 and Smad3 Levels in Cortex Renis in Mice Diabetic Nephropathy Model

目的:观察虎杖总蒽醌对糖尿病肾病(DN)模型小鼠肾脏皮质转化生长因子-β1(TGF-β1)、Smad3的影响。方法:将成模小鼠随机分为模型、开搏通、虎杖总蒽醌高、中、低剂量5组,每组17只,另外选取17只小鼠做为空白组。给药组分别ig高、中、低剂量的虎杖总蒽醌(400,200,100 mg.kg-1),共给药8周。运用免疫组化法,分别观察虎杖总蒽醌对DN模型肾脏皮质TGF-β1,Smad3的影响。结果:NH小鼠DN模型肾皮质TGF-β1,Smad3蛋白表达水平明显升高,而中剂量虎杖总蒽醌组NH小鼠肾皮质Smad3蛋白表达水平明显下降(IA为542.00±116.89)(P<0.05);虎杖总蒽醌高、中、低剂量组肾TGF-β1 IA值有降低的趋势,但无统计学意义。结论:TGF-β1,Smad3与DN形成有关;虎杖总蒽醌能显著抑制NH小鼠DN模型肾皮质Smad3蛋白表达。

Objective：To study the influence of polygonum cuspidatum anthraquinone（PCA） on transforming growth factor-petal（TGF-β1） and Smad3 levels in cortex renisin mice diabetic nephropathy（DN） model.Method：DN mouse were induced by STZ and randomized into high,middle and low close of PCA groups,positive control group and model group with 17 mousein each group.Another 17 mouse were chosen as normal control.The levels of PCA on TGF-β1 and Smad3 in cortex renis were assayed by immunohistochemistry method.Result：TGF-β1 and Smad3 levels in cortex renis from DN model were significantly increased,and Smad3 levels in cortex renis from middle close of PCA groups was significantly decreased.There was a downward tendency of TGF-β1 and Smad3 levels in cortex renis from high,middle and low dose of PCA groups,but no statistical significance.Conclusion：TGF-β1and Smad3 may involve in the occurrence of DN;PCA can inhibit Smad3 expression in cortex ren is from DN model.