摘要
目的:从信号转导通路上重要调节因子cAMP(环磷酸腺苷),PKA(蛋白激酶A)及PKC(蛋白激酶C)的角度,探讨抑郁大鼠模型受体后信号转导的变化及加味逍遥汤的干预作用。方法:将SD大鼠随机分为6个组,每组10只,即正常组、模型组、氯米帕明组(13.5 mg.kg-1)、加味逍遥汤高、中、低剂量组(生药11.7,5.85,2.925 g.kg-1)。造模同时各组按10 mL.kg-1体重给药,正常组、模型组给予等量的生理盐水,每日1次,连续24 d。末次给药后1 h,快速断头处死,在冰上剥离大脑,分离海马迅速放入冻存管,投入液氮中,随后置于-70℃低温冰箱保存备用。按ELISA试剂盒说明用酶标仪检测大鼠海马cAMP,PKA及PKC的含量变化。结果:与模型组比较,各组均能增加大鼠海马cAMP,PKA及PKC的含量,差异有显著性意义(P<0.05),加味逍遥汤各剂量组cAMP,PKA及PKC的含量增加,随剂量增加作用加强,与模型组比较差异有显著性意义(P<0.05);加味逍遥汤高、中剂量组与氯米帕明组相比cAMP,PKA及PKC含量差异没有显著性意义。加味逍遥汤高、中剂量组与低剂量组相比,差异有显著性意义(P<0.05)。结论:加味逍遥汤可能是通过调节受体后cAMP,PKA及PKC信号传导通路发挥抗抑郁作用。
Objective:To explore The effect of modified Jiawei Xiaoyao Tang on cyclic adenosine monophosphate(cAMP),protein kinase A(PKA) and protein kinase C(PKC) in rat hippocampus with depression.Method:Rats were randomly divided into six groups:control group,model group,clomipramine group(13.5 mg·kg-1),modified Jiawei Xiaoyao Tang of high,medium and low dose group(11.7,5.85,2.925 g·kg-1).Each group was administrated continuously for 24 ds.After 1 hour of administration,the hippocampus was separated rapidly into the freezing tube and placed in-70 ℃ refrigerator to detecte the level of cAMP,PKA and PKC by ELISA.Result:Compared with model group,each group of modified Jiawei Xiaoyao Tang significantly increased level of cAMP,PKA and PKC(P0.05) with dose-depandent manner.Compared with clomipramine group,high and medium dose showed the no significant differences.Conclusion:The anti-depression effect of modified Jiawei Xiaoyao Tang may be related to regulating the signal transduction of cAMP,PKA and PKC.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第4期162-164,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省建设中医强省科研课题(2010024)
关键词
加味逍遥汤
抑郁
环磷酸腺苷
蛋白激酶A
蛋白激酶C
modified Jiawei Xiaoyao Tang
depression
cyclic adenosine monophosphate
protein kinase A
protein kinase C