加味逍遥汤对抑郁大鼠海马cAMP,PKA,PKC的影响

Effect of Modified Jiawei Xiaoyao Tang on cAMP,PKA and PKC in Rat Hippocampus with Depression

目的:从信号转导通路上重要调节因子cAMP(环磷酸腺苷),PKA(蛋白激酶A)及PKC(蛋白激酶C)的角度,探讨抑郁大鼠模型受体后信号转导的变化及加味逍遥汤的干预作用。方法:将SD大鼠随机分为6个组,每组10只,即正常组、模型组、氯米帕明组(13.5 mg.kg-1)、加味逍遥汤高、中、低剂量组(生药11.7,5.85,2.925 g.kg-1)。造模同时各组按10 mL.kg-1体重给药,正常组、模型组给予等量的生理盐水,每日1次,连续24 d。末次给药后1 h,快速断头处死,在冰上剥离大脑,分离海马迅速放入冻存管,投入液氮中,随后置于-70℃低温冰箱保存备用。按ELISA试剂盒说明用酶标仪检测大鼠海马cAMP,PKA及PKC的含量变化。结果:与模型组比较,各组均能增加大鼠海马cAMP,PKA及PKC的含量,差异有显著性意义(P<0.05),加味逍遥汤各剂量组cAMP,PKA及PKC的含量增加,随剂量增加作用加强,与模型组比较差异有显著性意义(P<0.05);加味逍遥汤高、中剂量组与氯米帕明组相比cAMP,PKA及PKC含量差异没有显著性意义。加味逍遥汤高、中剂量组与低剂量组相比,差异有显著性意义(P<0.05)。结论:加味逍遥汤可能是通过调节受体后cAMP,PKA及PKC信号传导通路发挥抗抑郁作用。

Objective：To explore The effect of modified Jiawei Xiaoyao Tang on cyclic adenosine monophosphate（cAMP）,protein kinase A（PKA） and protein kinase C（PKC） in rat hippocampus with depression.Method：Rats were randomly divided into six groups：control group,model group,clomipramine group（13.5 mg·kg-1）,modified Jiawei Xiaoyao Tang of high,medium and low dose group（11.7,5.85,2.925 g·kg-1）.Each group was administrated continuously for 24 ds.After 1 hour of administration,the hippocampus was separated rapidly into the freezing tube and placed in-70 ℃ refrigerator to detecte the level of cAMP,PKA and PKC by ELISA.Result：Compared with model group,each group of modified Jiawei Xiaoyao Tang significantly increased level of cAMP,PKA and PKC（P0.05） with dose-depandent manner.Compared with clomipramine group,high and medium dose showed the no significant differences.Conclusion：The anti-depression effect of modified Jiawei Xiaoyao Tang may be related to regulating the signal transduction of cAMP,PKA and PKC.