葛根散对急性酒精性肝损伤小鼠肝微粒体CYP450含量及CYP2E1活性的影响

Effect of Gegen Powder on CYP450 Content of Liver Microsomal and Activity of CYP2E1 in Mice Acute Alcohol-induced Liver Injury

目的:考察经典解酒方葛根散对急性酒精性肝损伤小鼠肝微粒体细胞色素P450(CYP450)含量及细胞色素P450-2E1(CYP2E1)活性的影响。方法:实验小鼠分葛根散高、中、低剂量组、正常对照组和模型对照组,各给药组分别给予葛根散水煎剂(按生药量计,20,10,5 g.kg-1)ig,对照组代以等体积蒸馏水,每天1次,连续10 d;每天给药30 min后,模型对照组和各给药组以15 mL.kg-1 56%白酒ig,正常对照组代以等体积蒸馏水,以血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)及肝脏系数为参考指标,制备急性酒精性肝损伤小鼠模型;取小鼠肝组织检测葛根散对肝微粒体CYP450含量及CYP2E1活性的影响。结果:与正常对照组比较:模型对照组血清ALT、AST水平及肝脏系数显著升高(P<0.01),肝微粒体蛋白含量无明显变化,CYP450含量及CYP2E1活性显著提高(P<0.01)。与模型对照组比较:葛根散各剂量组血清ALT,AST水平及大剂量组肝脏系数呈明显降低(P<0.05或P<0.01),肝微粒体蛋白含量无明显变化,CYP450含量明显增多(P<0.01)且有剂量差异性(P<0.05),CYP2E1活性显著降低(P<0.01)且有剂量差异性(P<0.01)。结论:葛根散对急性酒精性肝损伤小鼠肝功能具有良性干预作用,能提高酒精性肝损伤小鼠肝微粒体CYP450含量和降低CYP2E1活性。

Objective：To observe the effects of Jiejiu recipes of Gegen powder on the cytochrome P450（CYP450） content of liver microsomal and the activity CYP2E1 in mice acute alcohol-induced liver injury model.Method：Mouse were divided into normal control group,model group and Gegen powder high,medium nad low dose groups.Gegen powder of 20,10,5 g·kg-1,was given once a day and continuing ten days.After 30 minutes of administration everyday,the mouse of model group and treatment groups were orally given 56% alcohol of 15 mL·kg-1 to establish acute alcohol-induced liver injury model,and the level of ALT,AST in serum and liver index were observed;Content of CYP450 and the activity of CYP2E1 were detected.Result：Compared with normal control group,the level of ALT,AST and liver index in model group had obvious enhance（P0.01）,the content of microsomal protein had no significant change,the content of CYP450 and the activity of CYP2E1 were significantly raised（P0.01）.Compared with model group,the level of ALT,AST and liver index in Gegen powder groups had obvious reduction（P0.05 or P0.01）,the content of microsomal protein had no significant change,the content of CYP450 was increased significantly（P0.01）,the activity of CYP2E1 was significantly decreased（P0.01） and there was a significant difference between different dosage groups（P0.05）.Conclusion：Gegen powder indicates the therapeutic effect on acute alcohol-induced liver injury,it can be contribute to increaseing the content of CYP450 and reduceing the activity of CYP2E1