清热化瘀方预处理对大鼠脑缺血再灌注损伤的神经保护作用

Neuron Protective Effect of Qingre Huayu Prescription Preconditioning on Cerebral Ischemia/Reperfusion Injury in Rats

目的:探讨清热化瘀方联合脑缺血预处理对大鼠脑缺血再灌注损伤的神经保护作用。方法:健康雄性SD大鼠160只,随机分为4组:假手术组、大脑中动脉缺血组(middle cerebral artery occlusion,MCAO)、脑缺血预处理组(brain ischemic preconditioning,BIP)、清热化瘀方组(Qingre Huayu prescription,QRHY),每组按照再灌注后12 h,1,2,3 d 4个时间点平均分为4个亚组,制备缺血预处理模型,评价脑缺血预处理对缺血再灌注大鼠脑组织病理形态学、脑梗死体积和神经细胞凋亡的影响及清热化瘀方的干预作用。结果:①光镜和电镜下观察到BIP组神经细胞的病理形态学改变较MCAO组明显减轻,且BIP组梗死面积明显小于MCAO组(P<0.05),QRHY组较BIP组上述损害进一步减轻(P<0.05)。②大鼠脑缺血再灌注后12 h,MCAO组细胞凋亡发生率较假手术组显著增加(P<0.01),1 d时达到高峰,以后时间点逐渐下降,但仍高于假手术组(P<0.01);BIP组各个时间点神经元凋亡发生率较MCAO组显著降低(P<0.05或P<0.01);QRHY组较BIP组神经细胞凋亡率进一步降低(P<0.05或P<0.01)。结论:清热化瘀方联合缺血预处理对大鼠脑缺血再灌注损伤有协同保护作用。

Objective：To investigate the neuron protective effect of Qingre Huayu prescription（QRHYP） preconditioning on focal cerebral ischemia/reperfusion（I/R） injury in rats.Method：160 male SD rats were randomly divided into four groups as following：sham-operation group,middle cerebral artery occlusion（MCAO） group,brain ischemia preconditioning（BIP） group and QRHY group.Each group was further divided into 4 subgroups according to 12 h,1 d,2 d and 3 d after I/R.The BIP model was established The pathomorphology of brain tissue,the apoptosis rate of neuron and infarct volume after I/R were evaliated.Result：①Light microscopy and electron microscopy showed that the change of pathomorphology in BIP group was more significantly improved than that in MCAO group.The infarct size of the BIP group was smaller than that of the MCAO group（P0.05）.QRHYP treatment could very markedly reduce those injury compared with BIP group（P0.05）.②The rate of apoptosis in MCAO increased markedly at 12 h after reperfusion than that of the sham group（P0.01）,and reached the peak at 1 d,then decreased continuously,but still higher than sham group（P0.01）.BIP could decrease the rate of apoptosis（P0.05 or P0.01）.QRHYP treatment could further lower apoptosis compared with BIP group（P0.05 or P0.01）.Conclusion：Qingre Huayu prescription precondtioning could have a synergistic neuron protective effect on I/R injury in rats.