摘要
目的:探讨内异方对实验性大鼠子宫内膜异位灶组织转化生长因子-β1(TGF-β1)的mRNA及蛋白表达的调控作用。方法:SD大鼠采用自身种植法建立子宫内膜异位症(EMS)模型,分为假手术组、模型组、内异方低、高剂量(含生药25.2,50.4 g.kg-1.d-1)、孕三烯酮对照组(2.5 mg.kg-1.d-1),ig 3周后,应用RT-PCR检测内异灶组织中TGF-β1 mRNA的表达,Western印迹检测内异灶组织TGF-β1的蛋白表达。结果:模型组TGF-β1 mRNA及蛋白表达水平明显高于假手术组(P<0.01),内异方组明显低于模型组(P<0.01),与孕三烯酮对照组相比无明显差异。结论:大鼠模型"异灶中TGF-β1 mRNA及蛋白的表达升高,内异方通过下调TGF-β1 mRNA及蛋白的表达,起到抗EMS的作用。
Objective：To investigate the controlling effects of Neiyi Fang on expression of mRNA and protein of（TGF-β1） in endometriosis（EMS） lesions of model rats.Method：The model of EMS was duplicated in SD rats,rats were divided into sham group,model group,low-dose of Neiyi Fang group（25.2 g·kg-1·d-1）,high-dose of Neiyi Fang group（50.4 g·kg-1·d-1） and western medicine（gestrinone） group（2.5 mg·kg-1·d-1）.RT-PCR was used to detect the expression of mRNA of TGF-β1 in EMS lesions.Western blot was used to detect the expression of protein of TGF-β1.Result：The level of expression of mRNA and protein of TGF-β1 in the model group was clearly higher than that in the sham group（P0.01）,while in the Neiyi Fang group,the level was clearly lower than that in the model group（P0.01）.The level in the Neiyi Fang group was no significant difference than in the western medicine group.Conclusion：The expression of mRNA and protein of TGF-β1 in EMS lesions of model rats were increased.Neiyi Fang resisted EMS through decreasing the expression of mRNA and protein of TGF-β1.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第4期227-229,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
上海市科委专项课题资助项目(09dz1976800)
