摘要
目的:建立并验证醋氨酚人肝细胞损伤模型,进一步研究甘草酸、甘草苷、异甘草素的保肝作用。方法:20 mmol.L-1醋氨酚作用HL-7702正常人肝细胞3 h,以四氮溴盐(MTT)为考察指标,通过阳性药物双环醇验证该细胞模型并获得量效参考值,进而研究不同质量浓度的甘草酸、甘草苷、异甘草素对人肝细胞损伤的保护作用。结果:醋氨酚人肝细胞损伤模型成功、方法可靠,甘草酸、甘草苷、异甘草素3种成分对人肝细胞醋氨酚损伤均具一定的保护作用。甘草酸的最低有效浓度为0.001 6μmol.L-1,保肝的最大效能为47.0%,达到最大效能的浓度为0.2μmol.L-1。甘草苷的最低有效浓度为0.4μmol.L-1,保肝的最大效能为22.9%,达到最大效能的浓度为2μmol.L-1。异甘草素的最低有效浓度为0.4μmol.L-1,保肝的最大效能为72.6%,达到最大效能的浓度为250μmol.L-1。结论:提供一种新的体外肝细胞损伤模型;在甘草用于保肝相关作用时,单纯以甘草酸、甘草苷作为质量评价指标是不够的。
Objective:To study the hepato-protective activity of glycyrrhizin,liquiritin and isoliquritigenin on a new HL-7702 cells injury model induced by acetaminophen.Method:HL-7702 cells injury induced by 20 mmol·L-1 acetaminophen for 3 hours were chosen as the liver cells injury model and the effect of glycyrrhizin,liquiritin and isoliquritigenin was assayed.The main index was MTT.Result:The new HL-7702 cells injury model induced by acetaminophen was successful.Glycyrrhizin,liquiritin and isoliquiritigenin had hepato-protective activity on the cell model.The minimum effective concentration of glycyrrhizin was 0.001 6 μmol·L-1.The hepato-protective maximum efficacy of glycyrrhizin was 47.0% at the concentration of 0.2 μmol·L-1.The minimum effective concentration of liquiritin was 0.4 μmol·L-1.The hepato-protective maximum efficacy of liquiritin was 22.9 % at the concentration of 2 μmol·L-1.The minimum effective concentration of isoliquritigenin was 0.4 μmol·L-1.The hepato-protective maximum efficacy of isoliquritigenin was 72.6%at the concentration of 250 μmol·L-1.Conclusion:The new HL-7702 cells injury model induced by acetaminophen could be applied on studying the hepato-protective effect of drugs.The hepato-protective activity of licorice has connection with not only glycyrrhizin,liquiritin but also isoliquritigenin.Therefore,it is not enough to treat glycyrrhizin,liquiritin as the quality evaluation index for licorice.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第4期245-248,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
甘草酸
甘草苷
异甘草素
醋氨酚人肝细胞损伤
保肝作用
glycyrrhizin
liquiritin
isoliquritigenin
liver cell injury
hepato-protective activity