甘草酸、甘草苷、异甘草素对醋氨酚人肝细胞损伤模型的保护作用比较

Hepato-protective Activity of Glycyrrhizin,Liquiritin and Isoliquiritigenin on HL-7702 Cells Injury Induced by Acetaminophen

目的:建立并验证醋氨酚人肝细胞损伤模型,进一步研究甘草酸、甘草苷、异甘草素的保肝作用。方法:20 mmol.L-1醋氨酚作用HL-7702正常人肝细胞3 h,以四氮溴盐(MTT)为考察指标,通过阳性药物双环醇验证该细胞模型并获得量效参考值,进而研究不同质量浓度的甘草酸、甘草苷、异甘草素对人肝细胞损伤的保护作用。结果:醋氨酚人肝细胞损伤模型成功、方法可靠,甘草酸、甘草苷、异甘草素3种成分对人肝细胞醋氨酚损伤均具一定的保护作用。甘草酸的最低有效浓度为0.001 6μmol.L-1,保肝的最大效能为47.0%,达到最大效能的浓度为0.2μmol.L-1。甘草苷的最低有效浓度为0.4μmol.L-1,保肝的最大效能为22.9%,达到最大效能的浓度为2μmol.L-1。异甘草素的最低有效浓度为0.4μmol.L-1,保肝的最大效能为72.6%,达到最大效能的浓度为250μmol.L-1。结论:提供一种新的体外肝细胞损伤模型;在甘草用于保肝相关作用时,单纯以甘草酸、甘草苷作为质量评价指标是不够的。

Objective：To study the hepato-protective activity of glycyrrhizin,liquiritin and isoliquritigenin on a new HL-7702 cells injury model induced by acetaminophen.Method：HL-7702 cells injury induced by 20 mmol·L-1 acetaminophen for 3 hours were chosen as the liver cells injury model and the effect of glycyrrhizin,liquiritin and isoliquritigenin was assayed.The main index was MTT.Result：The new HL-7702 cells injury model induced by acetaminophen was successful.Glycyrrhizin,liquiritin and isoliquiritigenin had hepato-protective activity on the cell model.The minimum effective concentration of glycyrrhizin was 0.001 6 μmol·L-1.The hepato-protective maximum efficacy of glycyrrhizin was 47.0% at the concentration of 0.2 μmol·L-1.The minimum effective concentration of liquiritin was 0.4 μmol·L-1.The hepato-protective maximum efficacy of liquiritin was 22.9 % at the concentration of 2 μmol·L-1.The minimum effective concentration of isoliquritigenin was 0.4 μmol·L-1.The hepato-protective maximum efficacy of isoliquritigenin was 72.6%at the concentration of 250 μmol·L-1.Conclusion：The new HL-7702 cells injury model induced by acetaminophen could be applied on studying the hepato-protective effect of drugs.The hepato-protective activity of licorice has connection with not only glycyrrhizin,liquiritin but also isoliquritigenin.Therefore,it is not enough to treat glycyrrhizin,liquiritin as the quality evaluation index for licorice.