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玉米ZmNAS1和ZmNAS2基因启动子的克隆与序列分析 被引量:1

Cloning and Sequence Analysis of the Promoters of ZmNAS1 and ZmNAS2 Genes in Maize
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摘要 根据已知玉米全基因组序列设计引物,采用PCR方法从基因组DNA中克隆两种玉米材料的Zm-NAS1和ZmNAS2基因启动子,利用生物信息学软件对启动子元件进行分析预测并比较在两种材料间差异。结果表明,ZmNAS1基因启动子在两种材料上差异明显,沈137较K12除一段574bp缺失外,他们之间还有24处单核苷酸多态性位点;两种材料间的ZmNAS2基因启动子无差异。ZmNAS1和ZmNAS2基因启动子均具有一些与光调控、激素诱导、响应逆境胁迫等有关的顺式作用元件,但数目存在一定差异;两个基因启动子上都有一定数目缺铁诱导元件的核心序列。根据启动子元件分析,初步判断ZmNAS1和ZmNAS2基因除受缺铁诱导表达外,还可能受到其他多种信号的共同调控,是一个复杂的调控过程。此外,ZmNAS1基因启动子在两种材料间差异明显,推测该基因在两种材料间受到的表达调控水平也不相同。 PCR reactions were carried out to clone promoters of ZmNAS1 and ZmNAS2 from genomic DNA using primers designed according to the whole genome sequence of maize.And then,cis-acting elements and the differences of the promoters between two cultivars(K12 and Shen137) of maize were analyzed by bioinformatics software.The results showed that compared to cultivar K12,Shen137 was lack of 574 bp sequences in its promoter,besides existed 24 SNPs.However,the promoters of ZmNAS2 had no difference between the two cultivars of maize.There were some cis-acting elements relevant to light regulating,hormone inducing and stress responding in promoters of ZmNAS1 and ZmNAS2 genes,and core sequences in elements associated with the iron-deficiency-response were also identified.According to the analysis of promoter elements,we make a pre-judgement that expression and regulation of ZmNAS1 and ZmNAS2 were a complex process,and the two genes were regulated by several signals besides iron-deficiency stress.In addition,the promoters of ZmNAS1 are significantly different in the two cultivars,which indicated that the expression and regulation of ZmNAS1 between the two cultivars are not same.
出处 《玉米科学》 CAS CSCD 北大核心 2012年第1期15-23,共9页 Journal of Maize Sciences
基金 山东省自然科学基金项目"玉米麦根酸代谢相关基因的分离"(Y2007D52) 教育部博士点基金项目"玉米MA代谢相关基因的克隆与序列分析"(20070434005) 山东省良种工程高产优质抗逆专用玉米新品种培育
关键词 玉米 ZmNAS1基因 ZmNAS2基因 启动子 克隆 序列分析 Maize ZmNAS1 ZmNAS2 Promoter Cloning Sequence analysis
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  • 1金亚波,韦建玉,王军.植物铁营养研究进展Ⅰ:生理生化[J].安徽农业科学,2007,35(32):10215-10219. 被引量:31
  • 2Romheld V, Marschner H. Evidence for a specic uptake sys- tem for iron phytosiderophore in roots of grasses[J]. Plant Physiology, 1986, 80: 175- 180.
  • 3Kawai S, Itoh K, Takagi S, et al. Studies on phytosiderophore.. biosynthesis of mugineic acid and 2-deoxymuginei acid in hordeum ulgare L. vat minorirugi[J]. Tetrahedron Lett, 1988, 29:1053 -1056.
  • 4Herbik A, Koch G, Mock H P, et al. Isolation, characteriza- tion and cDNA cloning of nicotianamine synthase from barley, a key enzyme for iron homeostasis in plants[J]. European Journal of Biochemistry, 1999, 265 : 231- 239.
  • 5Daichi Mizuno, Kyoko Higuchi, Tatsuya Sakamoto, et al. Three nicotianamine synthase genes isolated from maize are differentially regulated by iron nutritional status[J]. Plant Physiology, 2003, 132: 1989-1997.
  • 6魏春红,李毅.植物总DNA的提取[M].现代分子生物学实验,北京:高等教育出版社,2006.
  • 7杜玮南,孙红霞,方福德.单核苷酸多态性的研究进展[J].中国医学科学院学报,2000,22(4):392-394. 被引量:51
  • 8Joshi C P. An inspection of the domain between putative TA- TA box and translation start site in 79 plant genes[J]. Nucleic Acids Research, 1987, 15(16) : 6643-6653.
  • 9王维婷,张春庆,李鹏.玉米麦根酸测定方法研究[J].分析化学,2008,36(1):66-70. 被引量:5
  • 10Ogo Y, hal R N, Nakanishi H, et al. The rice bHLH pro- tein OslRO2 is an essential regulator of the genes involved in Fe uptake under Fe-defeient conditions[J]. Plant J, 2007, 51: 366-377.

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