栲树叶片SEM样品制备

SEM sample preparation for leaf epidermis of Castanopsis fargesii

栲树叶片下表皮覆盖着一层密集的薄壁盾状毛影响了对其叶表面特征的观察。经FAA固定→二甲苯和丙酮的混合液(1∶1)浸泡→恒温金属浴加温→超声波(50～60Hz,90W)振荡器→系列酒精浓度脱水处理后,叶表面薄壁盾状毛脱落,在SEM扫描电子显微镜下可观察到叶表皮形态结构(气孔器,细胞排列及近圆形的毛基)。此方法操作简便、效果明显,并且安全和无污染。

The lower epidermis of the leaves of Castanopsis fargesii（Fagaceae） covered densely with thin-walled peltate trichomes gave rise to difficulty for the observation of its micromorphological characteristics.The thin-walled peltate trichome was shed off when the leaves were treated with FAA solution and a mixture of dimethylbenzene and acetone（1∶1） ,followed by shaking in an ultrasonic wave bath（50-60 Hz,90 W） and rinsing with a series of ethanol concentration.The micromorphological characteristics of leaf epidermis could be shown clearly under the scanning electronic microscope（SEM） .This method was very effective and easy to operate,furthermore,it was safe and non-polluted.