番茄抗南方根结线虫种质材料NBS-LRR类抗病基因的检测

Detection of NBS-LRR type disease resistance gene in the resistance cultivars of tomato to Meloidogyne incognita

采用盆栽病土接种法,鉴定58份番茄(Solanum lycopersicum)种质材料对南方根结线虫(Meloido-gyne incognita)的抗性。结果表明:供试的58份番茄种质材料对南方根结线虫的抗病性存在明显差异,其中高抗材料1份,抗病材料3份,感病材料3份,高感材料51份;根据已知NBS-LRR类抗病基因NBS区域的保守系列,设计简并引物,对抗性材料基因组进行体外扩增,获得了约500bp的预期片段。

The resistance of 58 tomato Solanum lycopersicum materials to Meloidogyne incognita were studied by transplanting the tomatoes to disease soil in the pot. There were significant differences between 58 cultivars. The results showed that 1 cultivar was highly resistant, 3 were resistant, 3 were susceptible, 51 were highly susceptible to Meloidogyne incognita. Degenerate oligonucleotide primers were designed according to the conserved NBS motifs among some disease-resistant genes. These primers were used for the amplification of the disease-resistant gene analog （RGA） from the genome of resistance cultivars by polymerase chain reaction （PCR）, and about 500 bp-long PCR product was obtained.