一种复方中草药制剂对黄颡鱼非特异性免疫机能和抗病力的增强作用

Effect of complex Chinese herbs on non-specific immune function and disease resistance in yellow catfish (Pelteobagrus fulvidraco R.) by oral administration

根据供试黄颡鱼的鱼体质量添加中草药制剂剂量(0～1 000mg/kg)的不同,试验设6个组(对照、Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ),连续投喂30d后,测定黄颡鱼血液中白细胞的吞噬活性、血清中溶菌酶和补体活性;另采用嗜水气单胞菌(Aeromonas hydrophila)注射的方法进行人工攻毒,进行抗病力检测。结果表明,各供试组黄颡鱼血液中白细胞的吞噬活性均显著高于对照组。第Ⅰ组黄颡鱼血清中溶菌酶的活性与对照组之间没有显著性差异,而试验Ⅱ、Ⅲ、Ⅳ组和Ⅴ组黄颡鱼血清中溶菌酶的活性均显著高于对照组。第Ⅱ、Ⅲ、Ⅳ和第Ⅴ组黄颡鱼血清中补体活性与对照组之间存在显著差异,而第Ⅰ组与对照组之间无显著性差异。活菌攻毒的方式证明投喂复方中草药制剂能增强黄颡鱼对人工感染A.hydrophila活菌的抵抗力。从增强非特异性免疫机能与抗病力的效果考量,这种复方中草药制剂在黄颡鱼饵料中添加量以400～800mg/kg为宜。

The effects of the dietary complex Chinese herbs on non-specific immune function and disease resistance in yellow catfish （Pelteobagrusfulvidraco R. ） were studied. The yellow catfish were divided into 6 groups and were fed with feed supplemented with different ratios of complex Chinese herbs, 0 mg/kg（control group）, 200 mg/kg（group Ⅰ）, 400 mg/kg （group Ⅱ）, 600 mg/kg（group Ⅲ）, 800 mg/kg（group Ⅳ）and 1 000 mg/kg （group Ⅴ）, respectively. Thirty days later, the yellow catfish were blooded,and the leucocytes phagocytic activity, serum lysozyme activity, complement activity and disease resistance were monitored. The results showed that the phagocytic activity of the leucocytes were significantly higher than the control group （t-test, P（0.05）. The lysozyme activity of the serum in group Ⅲ, Ⅳand Ⅴwere significantly higher than the control group （t-test, P（0. 05）, but there were no significant differences between group Ⅰ, Ⅱ and the control group （t-test, P〉0. 05）. The complement activity of the serum in group Ⅱ,Ⅲ, Ⅳ and Ⅴwere significantly higher than the control group （t-test, P〈0. 05）, while there were no significant differences between group Ⅰand control group （t-test, P〉0.05）. The results showed that the best supplementation ratio of the complex Chinese herbs for the yellow catfish was about 400-800 mg/kg.