枯草芽孢杆菌产中性蛋白酶的发酵条件优化

Optimization on the fermentation conditions of proteinase neutral produced by bacillus subtilis

文章对一株枯草芽孢杆菌产中性蛋白酶的发酵条件进行了优化,采用单因素试验和正交试验确定了最优发酵条件为装液量60 ml/250 ml,转速200 r/min,初始pH值6.9,发酵周期60 h,接种量4%,发酵温度37℃。通过发酵条件的优化,酶活提高了311.7%。

This paper focused on the optimization of fermentation conditions of proteinase neutral produced by bacillus subtilis.Mono-factor experiment and orthogonal tests were undertaken to obtain the suitable fermentation conditions as follows： liquid level 60 ml/250 ml,shaker revolution 200 r/min,initial pH 6.9,fermentation period 60 h,inoculation size 4%,culture temperature 37 ℃.Under the optimized conditions,the maximum enzyme activity increased by 311.7%.