肠益康汤对脾虚泄泻小鼠肠道功能的保护作用

Protective Effects of Changyikang Decoction on Intestinal Function in Mice with Spleen Deficiency Induced Diarrhea

目的观察肠益康汤对脾虚动物肠道菌群失调及胃肠功能异常的调整作用。方法用大黄诱导脾虚泄泻小鼠模型,连续灌胃给予肠益康汤14d,采用镜检法测定肠道菌群水平,墨汁印迹法检测小肠推进率,电镜观察小肠黏膜。结果与模型组比较,肠益康汤高、中、低剂量组小鼠双歧杆菌和乳酸杆菌数量显著增加(P<0.05),肠益康高、中剂量组小鼠小肠碳末推进率显著降低(P<0.05),肠益康汤高、中、低剂量组小鼠小肠黏膜病理改变显著改善。结论肠益康汤能调节肠道菌群紊乱,抑制脾虚小鼠肠推进功能亢进,并对大黄致脾虚小鼠小肠黏膜的病理改变也有一定的保护作用。

Objective To investigate regulative actions of Changyikang Decoction,a traditional Chinese medicine prescription,against the imbalance of intestinal flora and gastrointestinal dysfunctions in mice with spleen deficiency.Methods The mouse mode of spleen deficiency induced diarrhea was induced by intragastric injection of rhubarb decoction.The mice with spleen deficiency were continuously given Changyikang Decoction via intragastric injection for 14 days.The intestinal flora was detected by using microscopy.The propulsion rate of ink in the intestine was observed by ink imprinting method.The histomorphological changes of intestinal mucosa were observed using transmission electron microscope.Results Compared with those in the model group,the amount of bifidobacterium and lactobacillus significantly increased in high-,middle-and low-dose Changyikang Decoction treated groups（P0.05）,and the propulsion rate of ink in the intestine significantly decreased in high-and middle-dose Changyikang Decoction treated groups（P0.05）,and the pathological changes of mouse intestinal mocosa were significantly alleviated in high-,middle-and low-dose Changyikang Decoction treated groups.Conclusion Changyikang Decoction can adjust the imbalance of intestinal flora,inhibit the propulsion movement of the intestine,and alleviate pathological changes of intestinal mucosa in mice with rhubarb induced spleen deficiency.