木瓜齐墩果酸和熊果酸等三萜酸类薄层色谱分离鉴别

Identification of Triterpene Acids Including Oleanolic Acid and Ursolic Acid in Fructus chaenomelis by Thin-layer Chromatography

目的建立木瓜齐墩果酸、熊果酸等三萜酸薄层色谱分离鉴别方法。方法将点于硅胶G板上的样品点浸入1%碘-二氯甲烷溶液中预处理,以环己烷-丙酮-乙酸乙酯-甲酸(9∶2∶1∶0.2)为展开剂。结果木瓜中熊果酸、齐墩果酸以及3-O-乙酰熊果酸能够被很好地分离和检识。结论薄层色谱法简便、可靠,使木瓜鉴别更具客观性和专属性。

Objective To establish efficient methods for identifying triterpene acids,including oleanolic acid and ursolic acid,in Fructus chaenomelis by thin-layer chromatography（TLC）.Methods The drops of samples on the plate of silica gel G were preprocessed with methylene dichloride solution containing 1% iodine,and then developed with the developer consisting of cyclohexane-acetone-acetic ether-methanoic acid（9∶2∶1∶0.2）.Results Oleanolic acid,ursolic acid,and 3-O-acetyl ursolic acid in Fructus chaenomelis could be identified clearly.Conclusion TLC is simple and reliable,and it has specificity and objectivity for identifying Fructus chaenomelis.